Dual mode competitive electrochemical immunoassay for dibutyl phthalate detection based on PEI functionalized nitrogen doped graphene-CoSe2/gold nanowires and thionine-Au@Pt core-shell
نویسندگان
چکیده
In this work, a sensitive dual mode competitive electrochemical immunosensor based on differential pulse voltammetry (DPV) and square wave (SWV) response modes for quantitative determination of dibutyl phthalate (DBP) was successfully established. According to diethylenetriamine (DETA) assisted hydrothermal strategy, novel N-doped graphene/CoSe2 nanobelt composite prepared. Afterwards, PEI functionalized NG-CoSe2 gold nanowires (AuNWs) were further compounded as preliminary signal amplification platform immunosensor. Thionine (Thi)-loaded [email protected] secondary antibody (Ab2) connected label. The immobilized coating antigen DBP-BSA the DBP compete limited binding sites. concentration can be determined by DPV detect Thi loaded protected]2 It also SWV current generated reaction system H2O2 o-phenylenediamine (o-PD) catalyzed with peroxidase like properties. Under optimum conditions, linear ranges 1 pg mL?1?1 ?g mL?1. And LOD 0.486 mL-1 0.711 (SWV), respectively. Besides, it exhibited outstanding selectivity, reproducibility, stability used detection in real samples.
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ژورنال
عنوان ژورنال: Sensors and Actuators B-chemical
سال: 2021
ISSN: ['0925-4005', '1873-3077']
DOI: https://doi.org/10.1016/j.snb.2020.129401